heavy metal regulation of plasma membrane h+-atpase gene expression in halophyte aeluropus littoralis

Authors

mohsen jam

abbas alemzadeh

ali mohammad tale

sara esmaeili-tazangi

abstract

the present study was conducted to find the effect of three heavy metals, ag, hg and pb on the expression level of a gene encoding plasma membrane h+-atpase in aeluropus littoralis. the experiment was laid out in a completely random design with three replications. the expression of the main gene was normalized to the expression of the housekeeping gene actin. two 259 and 187 bp fragments were amplified from plasma membrane h+-atpase and actin genes using specific primers in polymerase chain reactions. the results indicated that higher concentrations of all three heavy metals declined the expression of plasma membrane h+-atpase gene, whereas low concentrations changed the level of its transcript differently. a significant linear correlation was found between ag concentrations of aeluropus littoralis shoots and its external level; however, for hg and pb no correlations were observed. root weight decreased when plants were grown at both concentrations of ag and hg but increased at both concentrations of pb and nacl. maximum root weight was observed under lower levels of pb, while maximum shoot weight was observed under lower levels of hg. the greatest plant weight was obtained at low concentrations of hg and pb. taken together these results show the regulation of plasma membrane h+-atpase gene by heavy metals suggesting that aeluropus littoralis can be regarded as a phytoremediation accumulator of soils polluted with heavy metals.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Heavy metal regulation of plasma membrane H+-ATPase gene expression in halophyte Aeluropus littoralis

The present study was conducted to find the effect of three heavy metals, Ag, Hg and Pb on the expression level of a gene encoding plasma membrane H+-ATPase in Aeluropus littoralis. The experiment was laid out in a completely random design with three replications. The expression of the main gene was normalized to the expression of the housekeeping gene actin. Two 259 and 187 bp fragments were a...

full text

Up-regulation of plasma membrane H+-ATPase under salt stress may enable Aeluropus littoralis to cope with stress

Plasma membrane H+-ATPase is a major integral membrane protein with a role in various physiological processes including abiotic stress response. To study the effect of NaCl on the expression pattern of a gene encoding the plasma membrane H+-ATPase, an experiment was carried out in a completely random design with three replications. A pair of specific primers was designed based on the sequence o...

full text

NaCl regulation of plasma membrane H(+)-ATPase gene expression in a glycophyte and a halophyte.

NaCl regulation of plasma membrane H(+)-ATPase gene expression in the glycophyte tobacco (Nicotiana tabacum L. var Wisconsin 38) and the halophyte Atriplex nummularia L. was evaluated by comparison of organ-specific mRNA abundance using homologous cDNA probes encoding the ATPases of the respective plants. Accumulation of mRNA was induced by NaCl in fully expanded leaves and in roots but not in ...

full text

up-regulation of plasma membrane h+-atpase under salt stress may enable aeluropus littoralis to cope with stress

plasma membrane h+-atpase is a major integral membrane protein with a role in various physiological processes including abiotic stress response. to study the effect of nacl on the expression pattern of a gene encoding the plasma membrane h+-atpase, an experiment was carried out in a completely random design with three replications. a pair of specific primers was designed based on the sequence o...

full text

Up-regulation of plasma membrane H+-ATPase under salt stress may enable Aeluropus littoralis to cope with stress

Most plants encounter stress such as drought and salinity that adversely affect growth, development and crop productivity. The expression of the gene glutathione-s-transferases (GST) extends throughout various protective mechanisms in plants and allows them to adapt to unfavorable environmental conditions. GSTF1 (the first phi GSTFs class) gene expression patterns in the wheat cultivars Mahuti ...

full text

In Silico Characterization of Proteins Containing ARID-PHD Domain and Its Expression in Aeluropus littoralis Halophyte

Abiotic stresses are the most important factors that reduce the yield of crops. In this case, Bioinformatics analysis plays an important role to study genes, and their relatedness as well as prediction their function in response to abiotic stresses. Among all domains, ARID-PHD domain has been identified in plants and animals and has a very significant role in growth regulation, cell cycle, and ...

full text

My Resources

Save resource for easier access later


Journal title:
molecular biology research communications

Publisher: shiraz university press

ISSN 2322-181X

volume 3

issue 2 2014

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023